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Annexin V Binding Buffer Annexin V Binding Buffer

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RUO
Other Names
Annexin-V-Binding Buffer
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422201 100 mL SEK303
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Description

Annexin V Binding Buffer has been formulated for flow cytometric labeling of apoptotic cells with Annexin V reagents.  The buffer is provided as a ready-to-use solution.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Store between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Annexin V Binding Buffer is recommended for use with Annexin V reagents. Cells should be resuspended in Annexin V Binding Buffer at a concentration 1x106/ml and then combined with Annexin V reagents at the recommended volumes.

Application Notes

Annexin V Staining

  1. Wash cells twice with cold BioLegend Cell Staining Buffer (Cat. No. 420201) and then resuspend cells in Annexin V Binding Buffer (Cat. No. 422201) at a concentration of 1x106 cells/mL.
  2. Transfer 100 µL of cell suspension in 5 mL test tube.
  3. Add 5 µL of fluorochrome conjugated Annexin V.
  4. Stain with a viability dye, such as PI (Cat. No. 421301), 7-AAD (Cat. Nos. 420403 & 420404), or Helix NP dyes (Cat. Nos. 425301, 425303, & 425305), if desired. 
  5. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark.
  6. Add 400* µL of Annexin V Binding Buffer (Cat. No. 422201) to each tube. *For more concentrated samples, add a minimum of 200 µl of Annexin V Binding Buffer in this step.
  7. Analyze by flow cytometry.

For a better experience detecting apoptosis, we now recommend Apotracker™. Cell staining with Apotracker™ is Calcium independent. Thus, no special buffers are required, and the protocol can be shortened for single-step co-staining with other reagents.

Application References
  1. Vermes I, et al. 1995. J. Immunol. Methods 184:39.
  2. Soon PS, et al. 2013. Endocr Relat Cancer. 20:1. PubMed
  3. Turner JE, et al. 2013. J Exp Med. PubMed
  4. Lindner JM, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  5. Turner JE, et al. 2013 J. Exp Med. 210:2951. PubMed
  6. Beggs KM, et al. 2014. Toxicol Sci. 137:91. PubMed
  7. Miyazawa M, et al. 2014. Mol Biol Cell. 25:2116. PubMed
  8. Burbulla LF, et al. 2014. Cell Death Dis. 5:1180. PubMed
  9. de Vires M, 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  10. Sahin E, 2014. J. Immunol. 193:1717. PubMed
  11. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  12. Thakran S, 2015. Invest Ophthalmol Vis Sci. 56:177. PubMed
Product Citations
  1. Kitazawa M, et al. 2017. PLoS One. 10.1371/journal.pone.0169340. PubMed
  2. Liu M, et al. 2021. Mol Med Rep. 23:00. PubMed
  3. Ocklenburg T, et al. 2021. Sci Rep. 7199:11. PubMed
  4. Tsuji M 2014. Mol Biol Cell. 25:2116. PubMed
  5. Guo H, et al. 2016. Sci Rep. 6:37559. PubMed
  6. Chen Q, et al. 2022. Cell Rep. 39:110990. PubMed
  7. Clusmann J, et al. 2022. Cell Death Dis. 13:702. PubMed
  8. Hao J, et al. 2022. Cell Rep. 41:111804. PubMed
  9. Shahid AM, et al. 2022. PLoS One. 17:e0278209. PubMed
  10. Cappellesso F, et al. 2022. Nat Cancer. 3:1464. PubMed
  11. Muckenhuber M, et al. 2023. Am J Transplant. 23:84. PubMed
  12. Zhao Q, et al. 2023. Nat Commun. 14:735. PubMed
  13. Wang YH, et al. 2022. Sci Adv. 8:eabn6552. PubMed
  14. Vajda F, et al. 2022. Cells. 11:. PubMed
  15. Chu CY, et al. 2023. Sci Rep. 13:3943. PubMed
  16. Riera-Domingo C, et al. 2023. Sci Adv. 9:eadd5028. PubMed
  17. Bujarrabal-Dueso A, et al. 2023. Nat Struct Mol Biol. 30:475. PubMed
  18. Quintela M, et al. 2023. Clin Epigenetics. 15:63. PubMed
  19. Quintela M, et al. 2023. Br J Cancer. . PubMed
  20. McKenzie C, et al. 2023. Mol Ther Nucleic Acids. 32:603. PubMed
  21. Djeungoue–Petga MA, et al. 2019. Cell Death Dis. 1.069444444. PubMed
  22. Forika G, et al. 2021. Pathol Oncol Res. 27:1610048. PubMed
  23. Shrestha C,et al. 2017. PLoS One. 10.1371/journal.pone.0186169. PubMed
  24. Wang J, et al. 2021. ACS Nano. 15:14360. PubMed
  25. Lindner J, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  26. Turner J, et al. 2013. J Exp Med. 210:2951. PubMed
  27. Itkin T, et al. 2016. Nature. 532: 323-328. PubMed
  28. Hergott C, et al. 2016. Blood. 127: 2460 - 2471. PubMed
  29. Olelewe C, et al. 2022. iScience. 25:104340. PubMed
  30. Svoronos AA, et al. 2021. iScience. 24:103208. PubMed
  31. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  32. Kim Y, et al. 2020. J Neurochem. 155:177. PubMed
  33. Liu Y, et al. 2022. Cancer Discov. :. PubMed
  34. Wang M, et al. 2022. Immun Inflamm Dis. 10:e626. PubMed
  35. McNeal AS, et al. 2021. Elife. 10:. PubMed
  36. Lim JA, et al. 2018. BMC Cancer. 0.860416667. PubMed
  37. Venter C, et al. 2017. Cell Physiol Biochem. 43:1288. PubMed
  38. Terashima S, et al. 2017. J Radiat Res. 10.1093/jrr/rrx025. PubMed
  39. Wurzer H, et al. 2021. Front Immunol. 12:619069. PubMed
  40. Figueroa-Valdés AI, et al. 2021. Front Bioeng Biotechnol. 9:619930. PubMed
  41. Yoshioka H, et al. 2021. Front Cell Dev Biol. 9:621045. PubMed
  42. Soprano M, et al. 2016. PLoS One. 11: 0155970. PubMed
  43. Li L, et al. 2016. Cell Death Dis. 7:e2439. PubMed
  44. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  45. Moose DL, et al. 2020. Cell Rep. 30:3864. PubMed
  46. Dregalla RC, et al. 2021. Stem Cell Res Ther. 12:547. PubMed
  47. Sun V, et al. 2021. Front Immunol. 12:716661. PubMed
  48. Herman KD, et al. 2022. Front Immunol. 13:956991. PubMed
  49. Tian AL, et al. 2021. Cell Death Dis. 12:6. PubMed
  50. Vries M, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  51. Elakad O, et al. 2022. NPJ Precis Oncol. 6:52. PubMed
  52. Deng J, et al. 2021. Cell Death Dis. 12:978. PubMed
  53. Naaman H,et al. 2017. PLoS One. 10.1371/journal.pone.0187077. PubMed
  54. Hagan AS, et al. 2020. Development. 147:00:00. PubMed
  55. Hickman O, et al. 2016. Br J Cancer. 10.1038/bjc.2016.91. PubMed
  56. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  57. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  58. Feng S, et al. 2017. Cancer Sci. 108:12:00. PubMed
  59. Zhou L, et al. 2017. Tumour Biol. 39:1010428317694313. PubMed
  60. Wu J, et al. 2020. Immunity. 53:115. PubMed
  61. Zhang S, et al. 2020. Front Immunol. 1.757638889. PubMed
  62. Li P, et al. 2016. Mol Biol Cell. 27: 2542 - 2553. PubMed
  63. Luca E, et al. 2020. Mol Metab. 36:100967. PubMed
  64. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  65. Saint Fleur–Lominy S, et al. 2018. Cell Rep. 24:3045. PubMed
  66. Krishnasamy K,et al. 2017. Nat Commun. . 10.1038/s41467-017-00953-2. PubMed
  67. Herold N, et al. 2017. Nat Med. 23:256-263. PubMed
  68. Domenis R, et al. 2017. PLoS One. 10.1371/journal.pone.0169932. PubMed
  69. Xie S, et al. 2021. Sci Rep. 0.660416667. PubMed
  70. Burbulla L, et al. 2014. Cell Death Dis. 5:1180. PubMed
  71. Peiris T, et al. 2016. Development. 143: 1697 - 1709.. PubMed
  72. Wang P, et al. 2019. Nat Commun. 0.940972222. PubMed
  73. Celus W, et al. 2021. Cancer Immunol Res. Online ahead of print. PubMed
  74. Hönes J, et al. 2017. Sci Rep. . 10.1038/s41598-017-15866-9. PubMed
  75. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  76. Cheng CC, et al. 2020. Elife. 9:00. PubMed
  77. Leiendecker L, et al. 2020. EMBO Mol Med. 12:e12525. PubMed
  78. Matsuda K, et al. 2021. Cell Rep Methods. 1:100122. PubMed
  79. Carosi JM, et al. 2021. Autophagy. 17:2217. PubMed
  80. Jung E, et al. 2017. J Neurosci. 37:6837. PubMed
  81. Verma N, et al. 2020. Sci Adv. 6:00. PubMed
  82. Bartkowiak K, et al. 2021. Cancers (Basel). 13: . PubMed
  83. Thakran S, et al. 2015. Invest Ophthalmol Vis Sci . 56:177. PubMed
  84. Suarez S, et al. 2015. J Biol Chem. 290: 28311 - 28320. PubMed
  85. Deventhiran J, et al. 2016. J Virol. 90: 222 - 231. PubMed
  86. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  87. Litzenburger UM, et al. 2017. Genome Biol. 18:15. PubMed
  88. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  89. L Chang, Y Li, D Kaplan 2016. Sci Rep. 6:36862. PubMed
  90. Becker A, et al. 2016. Sci Rep. 6: 33847. PubMed
  91. Hopkins T, et al. 2015. Nucleic Acids Res. 10.1093/nar/gkv1515. PubMed
  92. Bersini S, et al. 2020. Life Sci Alliance. 3:e201900623. PubMed
  93. Lasrado N, et al. 2022. iScience. 25:103865. PubMed
  94. Textor A, et al. 2021. Cancers (Basel). 13:. PubMed
  95. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  96. Erdogan M, et al. 2022. Chem Biodivers. 19:e202200659. PubMed
  97. Molinar-Inglis O, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  98. Li B, et al. 2019. Oncol Rep. 41:608. PubMed
  99. Wang M, et al. 2018. Diabetologia. 62:335. PubMed
  100. Harro CM, et al. 2020. J Clin Invest. . PubMed
  101. Cabet E, et al. 2015. PLoS One. 10: 0137009. PubMed
  102. Pascual-Pasto G, et al. 2022. J Immunother Cancer. 10:. PubMed
  103. Hasselluhn MC, et al. 2019. Cell Death Dis. 0.726388889. PubMed
  104. Zhang S, et al. 2018. Sci Rep. 8:17066. PubMed
  105. Lee HW, et al. 2018. Am J Physiol Renal Physiol. 314:F280. PubMed
  106. Dong G, et al. 2020. Haematologica. . PubMed
  107. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  108. Bahl S, et al. 2021. Cell Death Dis. 12:469. PubMed
  109. Soon P, et al. 2013. Endocr Relat Cancer. 20:1. PubMed
  110. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  111. Su PL, et al. 2021. Lung Cancer. 158:137. PubMed
  112. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  113. Sun T, et al. 2019. Cancer Med. 2.457638889. PubMed
  114. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  115. Beggs K, et al. 2014. Toxicol Sci. 137:91. PubMed
  116. Guentsch A, et al. 2017. Mol Cell Biol. 37: e00236-16. PubMed
  117. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  118. Marton N, et al. 2017. Cellular and Molecular Life Sciences. 10.1007/s00018-017-2535-8. PubMed
  119. Kranz P, et al. 2017. Cell Death Dis. 8:e2986. PubMed
  120. Kojima N, et al. 2015. Anticancer Res. 35: 4493-4499. PubMed
  121. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  122. Pedro L, et al. 2019. Small. 15:e1900224. PubMed
  123. Rosenberger L, et al. 2019. Sci Rep. 0.835416667. PubMed
  124. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  125. Sahin E, et al. 2014. J Immunol. 193:1717. PubMed
  126. Quan M, et al. 2019. Oncol Rep. 41:3051. PubMed
  127. Di Franco S, et al. 2021. STAR Protoc. 2:100880. PubMed
  128. Jatho A, et al. 2022. Cells. 11:. PubMed
  129. Kim J, et al. 2019. PLoS One. 14:e0210248. PubMed
  130. Langsch S, et al. 2016. Cancer Res . 76: 4160 - 4169. PubMed
  131. Xiong R, et al. 2015. Mol Pharmacol. 88: 1045 - 1054. PubMed
  132. Vancsik T, et al. 2019. Cancer Med. 3.313888889. PubMed
  133. Vulin M, et al. 2022. Oncogene. :. PubMed
  134. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed
  135. Kitazawa M, et al. 2017. PLoS One. 10.1371/journal.pone.0169340. PubMed
  136. Liu M, et al. 2021. Mol Med Rep. 23:00. PubMed
  137. Ocklenburg T, et al. 2021. Sci Rep. 7199:11. PubMed
  138. Tsuji M 2014. Mol Biol Cell. 25:2116. PubMed
  139. Guo H, et al. 2016. Sci Rep. 6:37559. PubMed
  140. Chen Q, et al. 2022. Cell Rep. 39:110990. PubMed
  141. Clusmann J, et al. 2022. Cell Death Dis. 13:702. PubMed
  142. Hao J, et al. 2022. Cell Rep. 41:111804. PubMed
  143. Shahid AM, et al. 2022. PLoS One. 17:e0278209. PubMed
  144. Cappellesso F, et al. 2022. Nat Cancer. 3:1464. PubMed
  145. Muckenhuber M, et al. 2023. Am J Transplant. 23:84. PubMed
  146. Zhao Q, et al. 2023. Nat Commun. 14:735. PubMed
  147. Wang YH, et al. 2022. Sci Adv. 8:eabn6552. PubMed
  148. Vajda F, et al. 2022. Cells. 11:. PubMed
  149. Chu CY, et al. 2023. Sci Rep. 13:3943. PubMed
  150. Riera-Domingo C, et al. 2023. Sci Adv. 9:eadd5028. PubMed
  151. Bujarrabal-Dueso A, et al. 2023. Nat Struct Mol Biol. 30:475. PubMed
  152. Quintela M, et al. 2023. Clin Epigenetics. 15:63. PubMed
  153. Quintela M, et al. 2023. Br J Cancer. . PubMed
  154. McKenzie C, et al. 2023. Mol Ther Nucleic Acids. 32:603. PubMed
  155. Djeungoue–Petga MA, et al. 2019. Cell Death Dis. 1.069444444. PubMed
  156. Forika G, et al. 2021. Pathol Oncol Res. 27:1610048. PubMed
  157. Shrestha C,et al. 2017. PLoS One. 10.1371/journal.pone.0186169. PubMed
  158. Wang J, et al. 2021. ACS Nano. 15:14360. PubMed
  159. Lindner J, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  160. Turner J, et al. 2013. J Exp Med. 210:2951. PubMed
  161. Itkin T, et al. 2016. Nature. 532: 323-328. PubMed
  162. Hergott C, et al. 2016. Blood. 127: 2460 - 2471. PubMed
  163. Olelewe C, et al. 2022. iScience. 25:104340. PubMed
  164. Svoronos AA, et al. 2021. iScience. 24:103208. PubMed
  165. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  166. Kim Y, et al. 2020. J Neurochem. 155:177. PubMed
  167. Liu Y, et al. 2022. Cancer Discov. :. PubMed
  168. Wang M, et al. 2022. Immun Inflamm Dis. 10:e626. PubMed
  169. McNeal AS, et al. 2021. Elife. 10:. PubMed
  170. Lim JA, et al. 2018. BMC Cancer. 0.860416667. PubMed
  171. Venter C, et al. 2017. Cell Physiol Biochem. 43:1288. PubMed
  172. Terashima S, et al. 2017. J Radiat Res. 10.1093/jrr/rrx025. PubMed
  173. Wurzer H, et al. 2021. Front Immunol. 12:619069. PubMed
  174. Figueroa-Valdés AI, et al. 2021. Front Bioeng Biotechnol. 9:619930. PubMed
  175. Yoshioka H, et al. 2021. Front Cell Dev Biol. 9:621045. PubMed
  176. Soprano M, et al. 2016. PLoS One. 11: 0155970. PubMed
  177. Li L, et al. 2016. Cell Death Dis. 7:e2439. PubMed
  178. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  179. Moose DL, et al. 2020. Cell Rep. 30:3864. PubMed
  180. Dregalla RC, et al. 2021. Stem Cell Res Ther. 12:547. PubMed
  181. Sun V, et al. 2021. Front Immunol. 12:716661. PubMed
  182. Herman KD, et al. 2022. Front Immunol. 13:956991. PubMed
  183. Tian AL, et al. 2021. Cell Death Dis. 12:6. PubMed
  184. Vries M, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  185. Elakad O, et al. 2022. NPJ Precis Oncol. 6:52. PubMed
  186. Deng J, et al. 2021. Cell Death Dis. 12:978. PubMed
  187. Naaman H,et al. 2017. PLoS One. 10.1371/journal.pone.0187077. PubMed
  188. Hagan AS, et al. 2020. Development. 147:00:00. PubMed
  189. Hickman O, et al. 2016. Br J Cancer. 10.1038/bjc.2016.91. PubMed
  190. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  191. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  192. Feng S, et al. 2017. Cancer Sci. 108:12:00. PubMed
  193. Zhou L, et al. 2017. Tumour Biol. 39:1010428317694313. PubMed
  194. Wu J, et al. 2020. Immunity. 53:115. PubMed
  195. Zhang S, et al. 2020. Front Immunol. 1.757638889. PubMed
  196. Li P, et al. 2016. Mol Biol Cell. 27: 2542 - 2553. PubMed
  197. Luca E, et al. 2020. Mol Metab. 36:100967. PubMed
  198. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  199. Saint Fleur–Lominy S, et al. 2018. Cell Rep. 24:3045. PubMed
  200. Krishnasamy K,et al. 2017. Nat Commun. . 10.1038/s41467-017-00953-2. PubMed
  201. Herold N, et al. 2017. Nat Med. 23:256-263. PubMed
  202. Domenis R, et al. 2017. PLoS One. 10.1371/journal.pone.0169932. PubMed
  203. Xie S, et al. 2021. Sci Rep. 0.660416667. PubMed
  204. Burbulla L, et al. 2014. Cell Death Dis. 5:1180. PubMed
  205. Peiris T, et al. 2016. Development. 143: 1697 - 1709.. PubMed
  206. Wang P, et al. 2019. Nat Commun. 0.940972222. PubMed
  207. Celus W, et al. 2021. Cancer Immunol Res. Online ahead of print. PubMed
  208. Hönes J, et al. 2017. Sci Rep. . 10.1038/s41598-017-15866-9. PubMed
  209. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  210. Cheng CC, et al. 2020. Elife. 9:00. PubMed
  211. Leiendecker L, et al. 2020. EMBO Mol Med. 12:e12525. PubMed
  212. Matsuda K, et al. 2021. Cell Rep Methods. 1:100122. PubMed
  213. Carosi JM, et al. 2021. Autophagy. 17:2217. PubMed
  214. Jung E, et al. 2017. J Neurosci. 37:6837. PubMed
  215. Verma N, et al. 2020. Sci Adv. 6:00. PubMed
  216. Bartkowiak K, et al. 2021. Cancers (Basel). 13: . PubMed
  217. Thakran S, et al. 2015. Invest Ophthalmol Vis Sci . 56:177. PubMed
  218. Suarez S, et al. 2015. J Biol Chem. 290: 28311 - 28320. PubMed
  219. Deventhiran J, et al. 2016. J Virol. 90: 222 - 231. PubMed
  220. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  221. Litzenburger UM, et al. 2017. Genome Biol. 18:15. PubMed
  222. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  223. L Chang, Y Li, D Kaplan 2016. Sci Rep. 6:36862. PubMed
  224. Becker A, et al. 2016. Sci Rep. 6: 33847. PubMed
  225. Hopkins T, et al. 2015. Nucleic Acids Res. 10.1093/nar/gkv1515. PubMed
  226. Bersini S, et al. 2020. Life Sci Alliance. 3:e201900623. PubMed
  227. Lasrado N, et al. 2022. iScience. 25:103865. PubMed
  228. Textor A, et al. 2021. Cancers (Basel). 13:. PubMed
  229. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  230. Erdogan M, et al. 2022. Chem Biodivers. 19:e202200659. PubMed
  231. Molinar-Inglis O, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  232. Li B, et al. 2019. Oncol Rep. 41:608. PubMed
  233. Wang M, et al. 2018. Diabetologia. 62:335. PubMed
  234. Harro CM, et al. 2020. J Clin Invest. . PubMed
  235. Cabet E, et al. 2015. PLoS One. 10: 0137009. PubMed
  236. Pascual-Pasto G, et al. 2022. J Immunother Cancer. 10:. PubMed
  237. Hasselluhn MC, et al. 2019. Cell Death Dis. 0.726388889. PubMed
  238. Zhang S, et al. 2018. Sci Rep. 8:17066. PubMed
  239. Lee HW, et al. 2018. Am J Physiol Renal Physiol. 314:F280. PubMed
  240. Dong G, et al. 2020. Haematologica. . PubMed
  241. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  242. Bahl S, et al. 2021. Cell Death Dis. 12:469. PubMed
  243. Soon P, et al. 2013. Endocr Relat Cancer. 20:1. PubMed
  244. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  245. Su PL, et al. 2021. Lung Cancer. 158:137. PubMed
  246. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  247. Sun T, et al. 2019. Cancer Med. 2.457638889. PubMed
  248. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  249. Beggs K, et al. 2014. Toxicol Sci. 137:91. PubMed
  250. Guentsch A, et al. 2017. Mol Cell Biol. 37: e00236-16. PubMed
  251. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  252. Marton N, et al. 2017. Cellular and Molecular Life Sciences. 10.1007/s00018-017-2535-8. PubMed
  253. Kranz P, et al. 2017. Cell Death Dis. 8:e2986. PubMed
  254. Kojima N, et al. 2015. Anticancer Res. 35: 4493-4499. PubMed
  255. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  256. Pedro L, et al. 2019. Small. 15:e1900224. PubMed
  257. Rosenberger L, et al. 2019. Sci Rep. 0.835416667. PubMed
  258. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  259. Sahin E, et al. 2014. J Immunol. 193:1717. PubMed
  260. Quan M, et al. 2019. Oncol Rep. 41:3051. PubMed
  261. Di Franco S, et al. 2021. STAR Protoc. 2:100880. PubMed
  262. Jatho A, et al. 2022. Cells. 11:. PubMed
  263. Kim J, et al. 2019. PLoS One. 14:e0210248. PubMed
  264. Langsch S, et al. 2016. Cancer Res . 76: 4160 - 4169. PubMed
  265. Xiong R, et al. 2015. Mol Pharmacol. 88: 1045 - 1054. PubMed
  266. Vancsik T, et al. 2019. Cancer Med. 3.313888889. PubMed
  267. Vulin M, et al. 2022. Oncogene. :. PubMed
  268. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed

Antigen Details

Gene ID
NA

Related FAQs

How is your Annexin made and what sequence does it cover?

It is made in E. coli, covering human aa Met1-Asp320.

How does pH and staining temperature affect Annexin V-Phosphatidylserine binding?

Annexin-Phosphatidylserine binding is lost below pH 5.2 and with prolonged incubation over a temperature of 42°C.

Why do I need to use Annexin V Binding Buffer?

Annexin V binding requires the presence of calcium in the solution.  So, we provide Annexin V Binding Buffer (cat # 422201), which is optimized for the best performance of Annexin V staining.

Can I use RPMI during Annexin V staining?

It is best to follow protocol as described on the product data sheet. Moreover, RPMI 1640 has a relatively high concentration of phosphate and low calcium ion concentration, which negatively impacts Annexin binding to its target phosphatidylserine (PS). Measurement of cell death by using Annexin V may also be significantly affected by time of incubation on ice, calcium concentration, and type of medium.

Can I freeze Annexin V conjugates?

It should not be frozen as it will lead to loss of biological activity due to dimerization.

Is Annexin V suitable for conjugation with the Maxpar® kit for CyTOF®?

Maxpar® Labeling kits require the protein to be partially reduced, so the metal chelate can be introduced through an SH group in the hinge region of the reduced antibody. Human Annexin V contains only one Cysteine which was reported to be chemically inactive. Thus, the Maxpar® labeling protocol would not work with Annexin V, unless a free –SH group can be introduced to Annexin V.  For more information regarding SH-mediated conjugation of Annexin V please consult published papers such as this one.

Go To Top Version: 5    Revision Date: 03-15-2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Pricing & Availability
Regulatory Status
RUO
Other Names
Annexin-V-Binding Buffer
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422201 100 mL SEK303
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Description

Annexin V Binding Buffer has been formulated for flow cytometric labeling of apoptotic cells with Annexin V reagents.  The buffer is provided as a ready-to-use solution.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Store between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Annexin V Binding Buffer is recommended for use with Annexin V reagents. Cells should be resuspended in Annexin V Binding Buffer at a concentration 1x106/ml and then combined with Annexin V reagents at the recommended volumes.

Application Notes

Annexin V Staining

  1. Wash cells twice with cold BioLegend Cell Staining Buffer (Cat. No. 420201) and then resuspend cells in Annexin V Binding Buffer (Cat. No. 422201) at a concentration of 1x106 cells/mL.
  2. Transfer 100 µL of cell suspension in 5 mL test tube.
  3. Add 5 µL of fluorochrome conjugated Annexin V.
  4. Stain with a viability dye, such as PI (Cat. No. 421301), 7-AAD (Cat. Nos. 420403 & 420404), or Helix NP dyes (Cat. Nos. 425301, 425303, & 425305), if desired. 
  5. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark.
  6. Add 400* µL of Annexin V Binding Buffer (Cat. No. 422201) to each tube. *For more concentrated samples, add a minimum of 200 µl of Annexin V Binding Buffer in this step.
  7. Analyze by flow cytometry.

For a better experience detecting apoptosis, we now recommend Apotracker™. Cell staining with Apotracker™ is Calcium independent. Thus, no special buffers are required, and the protocol can be shortened for single-step co-staining with other reagents.

Application References
  1. Vermes I, et al. 1995. J. Immunol. Methods 184:39.
  2. Soon PS, et al. 2013. Endocr Relat Cancer. 20:1. PubMed
  3. Turner JE, et al. 2013. J Exp Med. PubMed
  4. Lindner JM, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  5. Turner JE, et al. 2013 J. Exp Med. 210:2951. PubMed
  6. Beggs KM, et al. 2014. Toxicol Sci. 137:91. PubMed
  7. Miyazawa M, et al. 2014. Mol Biol Cell. 25:2116. PubMed
  8. Burbulla LF, et al. 2014. Cell Death Dis. 5:1180. PubMed
  9. de Vires M, 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  10. Sahin E, 2014. J. Immunol. 193:1717. PubMed
  11. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  12. Thakran S, 2015. Invest Ophthalmol Vis Sci. 56:177. PubMed
Product Citations
  1. Kitazawa M, et al. 2017. PLoS One. 10.1371/journal.pone.0169340. PubMed
  2. Liu M, et al. 2021. Mol Med Rep. 23:00. PubMed
  3. Ocklenburg T, et al. 2021. Sci Rep. 7199:11. PubMed
  4. Tsuji M 2014. Mol Biol Cell. 25:2116. PubMed
  5. Guo H, et al. 2016. Sci Rep. 6:37559. PubMed
  6. Chen Q, et al. 2022. Cell Rep. 39:110990. PubMed
  7. Clusmann J, et al. 2022. Cell Death Dis. 13:702. PubMed
  8. Hao J, et al. 2022. Cell Rep. 41:111804. PubMed
  9. Shahid AM, et al. 2022. PLoS One. 17:e0278209. PubMed
  10. Cappellesso F, et al. 2022. Nat Cancer. 3:1464. PubMed
  11. Muckenhuber M, et al. 2023. Am J Transplant. 23:84. PubMed
  12. Zhao Q, et al. 2023. Nat Commun. 14:735. PubMed
  13. Wang YH, et al. 2022. Sci Adv. 8:eabn6552. PubMed
  14. Vajda F, et al. 2022. Cells. 11:. PubMed
  15. Chu CY, et al. 2023. Sci Rep. 13:3943. PubMed
  16. Riera-Domingo C, et al. 2023. Sci Adv. 9:eadd5028. PubMed
  17. Bujarrabal-Dueso A, et al. 2023. Nat Struct Mol Biol. 30:475. PubMed
  18. Quintela M, et al. 2023. Clin Epigenetics. 15:63. PubMed
  19. Quintela M, et al. 2023. Br J Cancer. . PubMed
  20. McKenzie C, et al. 2023. Mol Ther Nucleic Acids. 32:603. PubMed
  21. Djeungoue–Petga MA, et al. 2019. Cell Death Dis. 1.069444444. PubMed
  22. Forika G, et al. 2021. Pathol Oncol Res. 27:1610048. PubMed
  23. Shrestha C,et al. 2017. PLoS One. 10.1371/journal.pone.0186169. PubMed
  24. Wang J, et al. 2021. ACS Nano. 15:14360. PubMed
  25. Lindner J, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  26. Turner J, et al. 2013. J Exp Med. 210:2951. PubMed
  27. Itkin T, et al. 2016. Nature. 532: 323-328. PubMed
  28. Hergott C, et al. 2016. Blood. 127: 2460 - 2471. PubMed
  29. Olelewe C, et al. 2022. iScience. 25:104340. PubMed
  30. Svoronos AA, et al. 2021. iScience. 24:103208. PubMed
  31. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  32. Kim Y, et al. 2020. J Neurochem. 155:177. PubMed
  33. Liu Y, et al. 2022. Cancer Discov. :. PubMed
  34. Wang M, et al. 2022. Immun Inflamm Dis. 10:e626. PubMed
  35. McNeal AS, et al. 2021. Elife. 10:. PubMed
  36. Lim JA, et al. 2018. BMC Cancer. 0.860416667. PubMed
  37. Venter C, et al. 2017. Cell Physiol Biochem. 43:1288. PubMed
  38. Terashima S, et al. 2017. J Radiat Res. 10.1093/jrr/rrx025. PubMed
  39. Wurzer H, et al. 2021. Front Immunol. 12:619069. PubMed
  40. Figueroa-Valdés AI, et al. 2021. Front Bioeng Biotechnol. 9:619930. PubMed
  41. Yoshioka H, et al. 2021. Front Cell Dev Biol. 9:621045. PubMed
  42. Soprano M, et al. 2016. PLoS One. 11: 0155970. PubMed
  43. Li L, et al. 2016. Cell Death Dis. 7:e2439. PubMed
  44. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  45. Moose DL, et al. 2020. Cell Rep. 30:3864. PubMed
  46. Dregalla RC, et al. 2021. Stem Cell Res Ther. 12:547. PubMed
  47. Sun V, et al. 2021. Front Immunol. 12:716661. PubMed
  48. Herman KD, et al. 2022. Front Immunol. 13:956991. PubMed
  49. Tian AL, et al. 2021. Cell Death Dis. 12:6. PubMed
  50. Vries M, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  51. Elakad O, et al. 2022. NPJ Precis Oncol. 6:52. PubMed
  52. Deng J, et al. 2021. Cell Death Dis. 12:978. PubMed
  53. Naaman H,et al. 2017. PLoS One. 10.1371/journal.pone.0187077. PubMed
  54. Hagan AS, et al. 2020. Development. 147:00:00. PubMed
  55. Hickman O, et al. 2016. Br J Cancer. 10.1038/bjc.2016.91. PubMed
  56. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  57. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  58. Feng S, et al. 2017. Cancer Sci. 108:12:00. PubMed
  59. Zhou L, et al. 2017. Tumour Biol. 39:1010428317694313. PubMed
  60. Wu J, et al. 2020. Immunity. 53:115. PubMed
  61. Zhang S, et al. 2020. Front Immunol. 1.757638889. PubMed
  62. Li P, et al. 2016. Mol Biol Cell. 27: 2542 - 2553. PubMed
  63. Luca E, et al. 2020. Mol Metab. 36:100967. PubMed
  64. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  65. Saint Fleur–Lominy S, et al. 2018. Cell Rep. 24:3045. PubMed
  66. Krishnasamy K,et al. 2017. Nat Commun. . 10.1038/s41467-017-00953-2. PubMed
  67. Herold N, et al. 2017. Nat Med. 23:256-263. PubMed
  68. Domenis R, et al. 2017. PLoS One. 10.1371/journal.pone.0169932. PubMed
  69. Xie S, et al. 2021. Sci Rep. 0.660416667. PubMed
  70. Burbulla L, et al. 2014. Cell Death Dis. 5:1180. PubMed
  71. Peiris T, et al. 2016. Development. 143: 1697 - 1709.. PubMed
  72. Wang P, et al. 2019. Nat Commun. 0.940972222. PubMed
  73. Celus W, et al. 2021. Cancer Immunol Res. Online ahead of print. PubMed
  74. Hönes J, et al. 2017. Sci Rep. . 10.1038/s41598-017-15866-9. PubMed
  75. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  76. Cheng CC, et al. 2020. Elife. 9:00. PubMed
  77. Leiendecker L, et al. 2020. EMBO Mol Med. 12:e12525. PubMed
  78. Matsuda K, et al. 2021. Cell Rep Methods. 1:100122. PubMed
  79. Carosi JM, et al. 2021. Autophagy. 17:2217. PubMed
  80. Jung E, et al. 2017. J Neurosci. 37:6837. PubMed
  81. Verma N, et al. 2020. Sci Adv. 6:00. PubMed
  82. Bartkowiak K, et al. 2021. Cancers (Basel). 13: . PubMed
  83. Thakran S, et al. 2015. Invest Ophthalmol Vis Sci . 56:177. PubMed
  84. Suarez S, et al. 2015. J Biol Chem. 290: 28311 - 28320. PubMed
  85. Deventhiran J, et al. 2016. J Virol. 90: 222 - 231. PubMed
  86. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  87. Litzenburger UM, et al. 2017. Genome Biol. 18:15. PubMed
  88. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  89. L Chang, Y Li, D Kaplan 2016. Sci Rep. 6:36862. PubMed
  90. Becker A, et al. 2016. Sci Rep. 6: 33847. PubMed
  91. Hopkins T, et al. 2015. Nucleic Acids Res. 10.1093/nar/gkv1515. PubMed
  92. Bersini S, et al. 2020. Life Sci Alliance. 3:e201900623. PubMed
  93. Lasrado N, et al. 2022. iScience. 25:103865. PubMed
  94. Textor A, et al. 2021. Cancers (Basel). 13:. PubMed
  95. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  96. Erdogan M, et al. 2022. Chem Biodivers. 19:e202200659. PubMed
  97. Molinar-Inglis O, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  98. Li B, et al. 2019. Oncol Rep. 41:608. PubMed
  99. Wang M, et al. 2018. Diabetologia. 62:335. PubMed
  100. Harro CM, et al. 2020. J Clin Invest. . PubMed
  101. Cabet E, et al. 2015. PLoS One. 10: 0137009. PubMed
  102. Pascual-Pasto G, et al. 2022. J Immunother Cancer. 10:. PubMed
  103. Hasselluhn MC, et al. 2019. Cell Death Dis. 0.726388889. PubMed
  104. Zhang S, et al. 2018. Sci Rep. 8:17066. PubMed
  105. Lee HW, et al. 2018. Am J Physiol Renal Physiol. 314:F280. PubMed
  106. Dong G, et al. 2020. Haematologica. . PubMed
  107. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  108. Bahl S, et al. 2021. Cell Death Dis. 12:469. PubMed
  109. Soon P, et al. 2013. Endocr Relat Cancer. 20:1. PubMed
  110. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  111. Su PL, et al. 2021. Lung Cancer. 158:137. PubMed
  112. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  113. Sun T, et al. 2019. Cancer Med. 2.457638889. PubMed
  114. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  115. Beggs K, et al. 2014. Toxicol Sci. 137:91. PubMed
  116. Guentsch A, et al. 2017. Mol Cell Biol. 37: e00236-16. PubMed
  117. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  118. Marton N, et al. 2017. Cellular and Molecular Life Sciences. 10.1007/s00018-017-2535-8. PubMed
  119. Kranz P, et al. 2017. Cell Death Dis. 8:e2986. PubMed
  120. Kojima N, et al. 2015. Anticancer Res. 35: 4493-4499. PubMed
  121. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  122. Pedro L, et al. 2019. Small. 15:e1900224. PubMed
  123. Rosenberger L, et al. 2019. Sci Rep. 0.835416667. PubMed
  124. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  125. Sahin E, et al. 2014. J Immunol. 193:1717. PubMed
  126. Quan M, et al. 2019. Oncol Rep. 41:3051. PubMed
  127. Di Franco S, et al. 2021. STAR Protoc. 2:100880. PubMed
  128. Jatho A, et al. 2022. Cells. 11:. PubMed
  129. Kim J, et al. 2019. PLoS One. 14:e0210248. PubMed
  130. Langsch S, et al. 2016. Cancer Res . 76: 4160 - 4169. PubMed
  131. Xiong R, et al. 2015. Mol Pharmacol. 88: 1045 - 1054. PubMed
  132. Vancsik T, et al. 2019. Cancer Med. 3.313888889. PubMed
  133. Vulin M, et al. 2022. Oncogene. :. PubMed
  134. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed
  135. Kitazawa M, et al. 2017. PLoS One. 10.1371/journal.pone.0169340. PubMed
  136. Liu M, et al. 2021. Mol Med Rep. 23:00. PubMed
  137. Ocklenburg T, et al. 2021. Sci Rep. 7199:11. PubMed
  138. Tsuji M 2014. Mol Biol Cell. 25:2116. PubMed
  139. Guo H, et al. 2016. Sci Rep. 6:37559. PubMed
  140. Chen Q, et al. 2022. Cell Rep. 39:110990. PubMed
  141. Clusmann J, et al. 2022. Cell Death Dis. 13:702. PubMed
  142. Hao J, et al. 2022. Cell Rep. 41:111804. PubMed
  143. Shahid AM, et al. 2022. PLoS One. 17:e0278209. PubMed
  144. Cappellesso F, et al. 2022. Nat Cancer. 3:1464. PubMed
  145. Muckenhuber M, et al. 2023. Am J Transplant. 23:84. PubMed
  146. Zhao Q, et al. 2023. Nat Commun. 14:735. PubMed
  147. Wang YH, et al. 2022. Sci Adv. 8:eabn6552. PubMed
  148. Vajda F, et al. 2022. Cells. 11:. PubMed
  149. Chu CY, et al. 2023. Sci Rep. 13:3943. PubMed
  150. Riera-Domingo C, et al. 2023. Sci Adv. 9:eadd5028. PubMed
  151. Bujarrabal-Dueso A, et al. 2023. Nat Struct Mol Biol. 30:475. PubMed
  152. Quintela M, et al. 2023. Clin Epigenetics. 15:63. PubMed
  153. Quintela M, et al. 2023. Br J Cancer. . PubMed
  154. McKenzie C, et al. 2023. Mol Ther Nucleic Acids. 32:603. PubMed
  155. Djeungoue–Petga MA, et al. 2019. Cell Death Dis. 1.069444444. PubMed
  156. Forika G, et al. 2021. Pathol Oncol Res. 27:1610048. PubMed
  157. Shrestha C,et al. 2017. PLoS One. 10.1371/journal.pone.0186169. PubMed
  158. Wang J, et al. 2021. ACS Nano. 15:14360. PubMed
  159. Lindner J, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  160. Turner J, et al. 2013. J Exp Med. 210:2951. PubMed
  161. Itkin T, et al. 2016. Nature. 532: 323-328. PubMed
  162. Hergott C, et al. 2016. Blood. 127: 2460 - 2471. PubMed
  163. Olelewe C, et al. 2022. iScience. 25:104340. PubMed
  164. Svoronos AA, et al. 2021. iScience. 24:103208. PubMed
  165. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  166. Kim Y, et al. 2020. J Neurochem. 155:177. PubMed
  167. Liu Y, et al. 2022. Cancer Discov. :. PubMed
  168. Wang M, et al. 2022. Immun Inflamm Dis. 10:e626. PubMed
  169. McNeal AS, et al. 2021. Elife. 10:. PubMed
  170. Lim JA, et al. 2018. BMC Cancer. 0.860416667. PubMed
  171. Venter C, et al. 2017. Cell Physiol Biochem. 43:1288. PubMed
  172. Terashima S, et al. 2017. J Radiat Res. 10.1093/jrr/rrx025. PubMed
  173. Wurzer H, et al. 2021. Front Immunol. 12:619069. PubMed
  174. Figueroa-Valdés AI, et al. 2021. Front Bioeng Biotechnol. 9:619930. PubMed
  175. Yoshioka H, et al. 2021. Front Cell Dev Biol. 9:621045. PubMed
  176. Soprano M, et al. 2016. PLoS One. 11: 0155970. PubMed
  177. Li L, et al. 2016. Cell Death Dis. 7:e2439. PubMed
  178. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  179. Moose DL, et al. 2020. Cell Rep. 30:3864. PubMed
  180. Dregalla RC, et al. 2021. Stem Cell Res Ther. 12:547. PubMed
  181. Sun V, et al. 2021. Front Immunol. 12:716661. PubMed
  182. Herman KD, et al. 2022. Front Immunol. 13:956991. PubMed
  183. Tian AL, et al. 2021. Cell Death Dis. 12:6. PubMed
  184. Vries M, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  185. Elakad O, et al. 2022. NPJ Precis Oncol. 6:52. PubMed
  186. Deng J, et al. 2021. Cell Death Dis. 12:978. PubMed
  187. Naaman H,et al. 2017. PLoS One. 10.1371/journal.pone.0187077. PubMed
  188. Hagan AS, et al. 2020. Development. 147:00:00. PubMed
  189. Hickman O, et al. 2016. Br J Cancer. 10.1038/bjc.2016.91. PubMed
  190. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  191. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  192. Feng S, et al. 2017. Cancer Sci. 108:12:00. PubMed
  193. Zhou L, et al. 2017. Tumour Biol. 39:1010428317694313. PubMed
  194. Wu J, et al. 2020. Immunity. 53:115. PubMed
  195. Zhang S, et al. 2020. Front Immunol. 1.757638889. PubMed
  196. Li P, et al. 2016. Mol Biol Cell. 27: 2542 - 2553. PubMed
  197. Luca E, et al. 2020. Mol Metab. 36:100967. PubMed
  198. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  199. Saint Fleur–Lominy S, et al. 2018. Cell Rep. 24:3045. PubMed
  200. Krishnasamy K,et al. 2017. Nat Commun. . 10.1038/s41467-017-00953-2. PubMed
  201. Herold N, et al. 2017. Nat Med. 23:256-263. PubMed
  202. Domenis R, et al. 2017. PLoS One. 10.1371/journal.pone.0169932. PubMed
  203. Xie S, et al. 2021. Sci Rep. 0.660416667. PubMed
  204. Burbulla L, et al. 2014. Cell Death Dis. 5:1180. PubMed
  205. Peiris T, et al. 2016. Development. 143: 1697 - 1709.. PubMed
  206. Wang P, et al. 2019. Nat Commun. 0.940972222. PubMed
  207. Celus W, et al. 2021. Cancer Immunol Res. Online ahead of print. PubMed
  208. Hönes J, et al. 2017. Sci Rep. . 10.1038/s41598-017-15866-9. PubMed
  209. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  210. Cheng CC, et al. 2020. Elife. 9:00. PubMed
  211. Leiendecker L, et al. 2020. EMBO Mol Med. 12:e12525. PubMed
  212. Matsuda K, et al. 2021. Cell Rep Methods. 1:100122. PubMed
  213. Carosi JM, et al. 2021. Autophagy. 17:2217. PubMed
  214. Jung E, et al. 2017. J Neurosci. 37:6837. PubMed
  215. Verma N, et al. 2020. Sci Adv. 6:00. PubMed
  216. Bartkowiak K, et al. 2021. Cancers (Basel). 13: . PubMed
  217. Thakran S, et al. 2015. Invest Ophthalmol Vis Sci . 56:177. PubMed
  218. Suarez S, et al. 2015. J Biol Chem. 290: 28311 - 28320. PubMed
  219. Deventhiran J, et al. 2016. J Virol. 90: 222 - 231. PubMed
  220. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  221. Litzenburger UM, et al. 2017. Genome Biol. 18:15. PubMed
  222. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  223. L Chang, Y Li, D Kaplan 2016. Sci Rep. 6:36862. PubMed
  224. Becker A, et al. 2016. Sci Rep. 6: 33847. PubMed
  225. Hopkins T, et al. 2015. Nucleic Acids Res. 10.1093/nar/gkv1515. PubMed
  226. Bersini S, et al. 2020. Life Sci Alliance. 3:e201900623. PubMed
  227. Lasrado N, et al. 2022. iScience. 25:103865. PubMed
  228. Textor A, et al. 2021. Cancers (Basel). 13:. PubMed
  229. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  230. Erdogan M, et al. 2022. Chem Biodivers. 19:e202200659. PubMed
  231. Molinar-Inglis O, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  232. Li B, et al. 2019. Oncol Rep. 41:608. PubMed
  233. Wang M, et al. 2018. Diabetologia. 62:335. PubMed
  234. Harro CM, et al. 2020. J Clin Invest. . PubMed
  235. Cabet E, et al. 2015. PLoS One. 10: 0137009. PubMed
  236. Pascual-Pasto G, et al. 2022. J Immunother Cancer. 10:. PubMed
  237. Hasselluhn MC, et al. 2019. Cell Death Dis. 0.726388889. PubMed
  238. Zhang S, et al. 2018. Sci Rep. 8:17066. PubMed
  239. Lee HW, et al. 2018. Am J Physiol Renal Physiol. 314:F280. PubMed
  240. Dong G, et al. 2020. Haematologica. . PubMed
  241. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  242. Bahl S, et al. 2021. Cell Death Dis. 12:469. PubMed
  243. Soon P, et al. 2013. Endocr Relat Cancer. 20:1. PubMed
  244. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  245. Su PL, et al. 2021. Lung Cancer. 158:137. PubMed
  246. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  247. Sun T, et al. 2019. Cancer Med. 2.457638889. PubMed
  248. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  249. Beggs K, et al. 2014. Toxicol Sci. 137:91. PubMed
  250. Guentsch A, et al. 2017. Mol Cell Biol. 37: e00236-16. PubMed
  251. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  252. Marton N, et al. 2017. Cellular and Molecular Life Sciences. 10.1007/s00018-017-2535-8. PubMed
  253. Kranz P, et al. 2017. Cell Death Dis. 8:e2986. PubMed
  254. Kojima N, et al. 2015. Anticancer Res. 35: 4493-4499. PubMed
  255. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  256. Pedro L, et al. 2019. Small. 15:e1900224. PubMed
  257. Rosenberger L, et al. 2019. Sci Rep. 0.835416667. PubMed
  258. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  259. Sahin E, et al. 2014. J Immunol. 193:1717. PubMed
  260. Quan M, et al. 2019. Oncol Rep. 41:3051. PubMed
  261. Di Franco S, et al. 2021. STAR Protoc. 2:100880. PubMed
  262. Jatho A, et al. 2022. Cells. 11:. PubMed
  263. Kim J, et al. 2019. PLoS One. 14:e0210248. PubMed
  264. Langsch S, et al. 2016. Cancer Res . 76: 4160 - 4169. PubMed
  265. Xiong R, et al. 2015. Mol Pharmacol. 88: 1045 - 1054. PubMed
  266. Vancsik T, et al. 2019. Cancer Med. 3.313888889. PubMed
  267. Vulin M, et al. 2022. Oncogene. :. PubMed
  268. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed

Antigen Details

Gene ID
NA

Related FAQs

How is your Annexin made and what sequence does it cover?

It is made in E. coli, covering human aa Met1-Asp320.

How does pH and staining temperature affect Annexin V-Phosphatidylserine binding?

Annexin-Phosphatidylserine binding is lost below pH 5.2 and with prolonged incubation over a temperature of 42°C.

Why do I need to use Annexin V Binding Buffer?

Annexin V binding requires the presence of calcium in the solution.  So, we provide Annexin V Binding Buffer (cat # 422201), which is optimized for the best performance of Annexin V staining.

Can I use RPMI during Annexin V staining?

It is best to follow protocol as described on the product data sheet. Moreover, RPMI 1640 has a relatively high concentration of phosphate and low calcium ion concentration, which negatively impacts Annexin binding to its target phosphatidylserine (PS). Measurement of cell death by using Annexin V may also be significantly affected by time of incubation on ice, calcium concentration, and type of medium.

Can I freeze Annexin V conjugates?

It should not be frozen as it will lead to loss of biological activity due to dimerization.

Is Annexin V suitable for conjugation with the Maxpar® kit for CyTOF®?

Maxpar® Labeling kits require the protein to be partially reduced, so the metal chelate can be introduced through an SH group in the hinge region of the reduced antibody. Human Annexin V contains only one Cysteine which was reported to be chemically inactive. Thus, the Maxpar® labeling protocol would not work with Annexin V, unless a free –SH group can be introduced to Annexin V.  For more information regarding SH-mediated conjugation of Annexin V please consult published papers such as this one.

Go To Top Version: 5    Revision Date: 03-15-2021

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